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. 2022 Dec 15;146(2):612–628. doi: 10.1093/brain/awac358

Figure 2.

Figure 2

Neuronal activity of grafted neurons in the ipsilateral striatum of the HIE-injured brain. (A) The strategy for electrophysiological recording of grafted human neurons. (B–G) Typical traces of injection current- (B and E) or blue light- (C and F) induced AP and spontaneous AP (D and G) of grafted neurons 2 and 6 MPT. The numbers in the upper right corner represent the numbers of neurons showing AP among recorded cells. (H) The current (0–100 pA, step 10 pA, duration 400 ms) induced spike numbers of grafted neurons 2 MPT (n = 12) or 6 MPT (n = 9). (I) Quantification of the AP ratio showed a significantly increased response to blue light stimulation at 6 months (n = 11) compared to 2 months (n = 11) after cell transplantation. (J–L) Statistical data show increased membrane capacitance (n = 14 for 2 months, n = 7 for 6 months) (J), decreased membrane resistance, recorded cell number (n = 13 for 2 months, n = 6 for 6 months) (K) and increased resting membrane potentials (n = 12 for 2 months, n = 11 for 6 months) of grafted neurons at 6 MPT compared with 2 MPT. (L). n = 3 independent experiments. Data are presented as the mean ± SEM. Student’s t-test, **P < 0.01, ***P < 0.001. AP = action potential; Cm = membrane capacitance; CPu = caudate putamen; Rm = membrane resistance; RMP = resting membrane potential.