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. 2022 Dec 15;146(2):612–628. doi: 10.1093/brain/awac358

Figure 6.

Figure 6

Synaptic regulation of grafted striatal neurons in the HIE-injured brain. (A) The strategy for whole-cell patch-clamp recording of synaptic regulation from the host striatum or cortex to intrastriatally grafted striatal neurons. (B and C) Typical traces of sEPSCs and sIPSCs from grafted neurons at 2 and 6 MPT. The numbers in the upper right corner represent the numbers of neurons showing spikes of sIPSCs and sEPSCs among recorded cells. (D–G) The frequency (D and F) and amplitude (E and G) of sEPSCs and sIPSCs at 2 (n = 9 for sEPSCs, n = 9 for sIPSCs) and 6 MPT (n = 7 for sIPSCs, n = 8 for sEPSCs) are plotted. (H–K) Cumulative distributions of the interevent intervals (H and J) and amplitude (I and K) of sEPSCs and sIPSCs for (B) and (C), respectively. (L and M) Typical traces of single pulse-induced EPSCs in grafted cells responding to optogenetic activation of host cortical neurons from M1 and M2 brain regions 6 MPT (top), which are blocked by CNQX (bottom). The amplitude of light-induced EPSC is plotted in (M) (n = 10). (N) Typical traces of paired pulse-induced EPSCs in grafted cells responding to optogenetic activation of host cortical neurons in M1 and M2 at 6 MPT (top), which are blocked by CNQX (bottom). (O) The paired pulse ratio (PPR = 2nd EPSC/1st EPSC) is plotted (n = 9). Data are presented as the mean ± SEM, *P < 0.05, **P < 0.01.