FIGURE 1.

Microglial phagocytosis of synaptosomes is P2Y₆R dependent. (a) Microglial uptake of synaptosomes was measured by flow cytometry. Microglial events were gated (left) by forward scatter (FSC‐A) and side scatter (SSC‐A), and gated events were analyzed for red fluorescence in FL3 in terms of fluorescence gate shift (middle) or mean fluorescence shift (right). (b) pHrodo‐labeled synaptosome uptake by BV2 microglia (n = 5) when treated with 500 nM MRS2578, a P2Y₆R inhibitor. (c) pHrodo‐labeled synaptosome uptake by P2ry6 ^+/+ and P2ry6 ^−/− primary mouse microglia (n = 4). Each point represents one individual experiment with three technical replicates. Statistical comparisons were made via a one‐sample t test. Error bars represent ±SEM, **p < 0.01, *p < 0.05