FIGURE 6.

Macrophages within the aged fat transplant contribute to atherogenesis. Aged WT donor mice were treated with clodronate liposomaes according to the experimental schema described in Figure 5a. (a) Representative images of H&E or Mac2 stained right carotid artery, aortic root and BCA sections of Ldlr ^−/− mice that received sham surgery or visceral fat transplants from control liposome treated (Donor‐L) or clodronate‐liposome treated (Donor‐C) aged donor mice. (b–d) The quantifications of lesion area, necrotic area, Mac2 positive area, and the percentage of Mac2 positive area in each section of (b) right carotid artery, (c) aortic root, and (d) BCA are shown (n = 8/group). Note: no necrosis was observed in carotid artery. Results are presented as means ± SEM. Two‐way ANOVA followed by Tukey's post hoc test was used for statistical analysis. p values indicate the main effect between groups of Donor‐L and Donor‐C.