Figure 3.

Effect of inflammation and senescence on mitochondrial transfer. (A) Mitochondrial transfer (measured using flow cytometry) from chondrocytes (CH), CH pretreated with tumor necrosis factor α (TNF-α) to induce inflammation (tCH), and CH pretreated with mitomycin C to induce senescence (sCH) to mesenchymal stromal cells (MSCs) and MSCs pretreated with mitomycin C to induce senescence (sMSC) tends to increase when CH are senescent (sCH). (B) Mitochondrial transfer (measured using flow cytometry) from sMSCs and MSC to CH tends to be increased in case of sMSC and sCH. Simulating an inflammatory environment using TNF-α in CH did not influence the speed and magnitude of mitochondrial transfer. Inflammation and senescence did not significantly change mitochondrial transfer. Error bars show standard deviations.