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. 2023 Feb 13;18(2):e0281688. doi: 10.1371/journal.pone.0281688

Fig 4. Frequencies and absolute numbers of ILC in the blood of hidradenitis suppurativa (HS) patients on anti-TNF treatment or non-biologics, compared to healthy controls (HC).

Fig 4

PBMC were analysed by flow cytometry for frequencies of ILC types 1 (ILC1), 2 (ILC2) and 3 (ILC3) and total ILC. ILC were defined as CD45+CD127+ lineage-negative, with ILC1 defined as CD117-CD161+, ILC2 defined as CD294+ and ILC3 defined as CD117+ NKP44+/-. Lineage ‘cocktail’ included antibodies specific for CD1a, CD3, CD11c, CD14, CD19, CD34, CD94, CD123, CD303, FCεRI, TCRαβ and TCRγδ. Precision count beads were used to calculate cell numbers. (a-d) average (± SEM) frequency of ILC1, ILC2 and ILC3 (% of lineage-negative cells (a-c) or total ILC (% of leukocytes) (d) in blood of healthy controls (HC, n = 10), and HS patients on non-biologic therapy (HS, n = 21) or anti-TNF (HS+αTNF, n = 7). (e-h) cell counts (± SEM) of ILC1, ILC2, ILC3 and total ILC per ml of blood in healthy controls (HC, n = 10), and HS patients on non-biologic therapy (HS, n = 15) or anti-TNF (HS+αTNF, n = 7). **P<0.01, ***P<0.001 using Kruskal-Wallis test with Dunn’s multiple comparisons.