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. 2023 Feb 13;18(2):e0281610. doi: 10.1371/journal.pone.0281610

Fig 1. Screening of growth factors and miRNAs that promote CM cell cycle re-entry.

Fig 1

(A) Representative photomicrographs showing EdU immunostaing after treating growth factors and/or miRNAs for 72 hours. Nuclei were stained by Dapi (blue), CMs by a-actinin (green) and cycling cells by EdU (red). Bar = 100μM. (B) Effects of indicated growth factors on CM EdU incorporation after 72 hours treatment in NRVMs. (C) Impact of overexpression of indicated miRNAs on CM EdU incorporation after 72 hours transfection in NRVMs. NRVMs were exposed to EdU for 24 hours. Statistics: Fold change of EdU+ NRVMs was normalized to control group; *p<0.05 in One-way ANOVA analysis followed by Tukeys’ Test; n = 3 for each group.