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. 2023 Feb 13;18(2):e0281610. doi: 10.1371/journal.pone.0281610

Fig 2. Effect of KDM4D and miR-199 on NRVM cell cycle activity.

Fig 2

(A) Representative western blot of cell lysates from NRVMs treated with 5 days of Ad-KDM4D and 3 days of miR-199 (representative data from one of the three replicates). (B) Quantification of pYap1/Yap1 ratio from western blot. (C) RT-qPCR analysis shows the expression of cell cycle genes after 5 days of Ad-KDM4D and 3 days of miR-199 treatment. (D) The effect of FGF on KDM4D- and miR-199- stimulated cell cycle gene expression after 3 days (RT-qPCR assay). (E) EdU+ incorporation in NRVMs treated with or without Ad-KDM4D, miR-199, and FGF. (F) pH3+ immunostaining of NRVMs treated with indicated interventions. (G) Total cell number was measured by hemocytometer on NRVMs treated as indicated. (H) Representative photomicrographs showing EdU/phospho-H3 immunostaing after different treatments. EdU, bar = 100μM; phospho-H3, bar = 200μM. Statistics: * showed statistical significance at p<0.05 vs control (without miR-199, Ad-KDM4D, and FGF); # represented statistical significance at p<0.05 vs miR-199; $ mean statistical significance at p<0.05 vs Ad-KDM4D; & showed statistical significance at p<0.05 vs miR-199+Ad-KDM4D. One-way ANOVA followed by Tukeys’ Test; n = 3 for all the experiments.