Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Candida albicans) |
REP1 | Candida Genome Database | CR_00,140 W | |
| Gene (Candida tropicalis) |
REP1 | Candida Genome Database | CTRG1_01069 | |
| Gene (Candida parapsilosis) |
REP1 | Candida Genome Database | CPAR2_800080 | |
| Gene (Candida albicans) |
CGA1 | Candida Genome Database | C1_13,320 C | |
| Gene (Candida tropicalis) |
CGA1 | Candida Genome Database | CTRG1_03606 | |
| Gene (Candida parapsilosis) |
CGA1 | Candida Genome Database | CPAR2_202690 | |
| Strain, strain background (Escherichia coli) | DH5α | Tsingke | TSC-C01 | |
| Strain, strain background (Escherichia coli) | BL21 | Tsingke | TSC-C06 | |
| Antibody | Anti-His (mouse monoclonal) | MBL | D291-3 | 1:5000 |
| Antibody | Anti-c-Myc (rabbit polyclonal) | Sigma-Aldrich | C3956 | 1:5000 |
| Antibody | Anti-FLAG (rabbit polyclonal) | Sigma-Aldrich | F7425 | 1:3000 |
| Antibody | Anti-tubulin (rat monoclonal) |
BioRad | MCA78G | 1:3000 |
| Antibody | Anti-mouse IgG, (HRP-linked, mouse polyclonal) | BioRad | 1706516 | 1:3000 |
| Antibody | Anti-rat IgG (HRP-linked, rat polyclonal) | CST | 7077 | 1:5000 |
| Antibody | Anti-rabbit IgG, (HRP-linked, rabbit polyclonal) | Sigma-Aldrich | A3687 | 1:5000 |
| Sequence-based reagent | pBA1-F | This paper | PCR primers | CAACAACAAATACAAAAACAAAGATCT |
| Sequence-based reagent | pBA1-R | This paper | PCR primers | ATACGACTCACATAGGGCGAATTGGGTACC |
| Sequence-based reagent | pET28a-F | This paper | PCR primers | CTCACAGAGAACAGATTGGTGGATCC |
| Sequence-based reagent | pET28a-R | This paper | PCR primers | TCAGTGGTGGTGGTGGTGGTGCTCGA |
| Peptide, recombinant protein | Rep1ΔAD | This paper | purified from E. coli BL21 | |
| Peptide, recombinant protein | Rep1ΔAD Y526A | This paper | purified from E. coli BL21 | |
| Peptide, recombinant protein | GST-Cga1 | This paper | purified from E. coli BL21 | |
| Commercial assay or kit | RNeasy Minikit | Qiagen | 74204 | |
| Commercial assay or kit | GFP-Trap | Chromotek | Gta-20 | |
| Chemical compound, drug | D-Galactose | Sigma-Aldrich | G5388 | |
| Chemical compound, drug | N-Acetyl-D-glucosamine | MP | 1727589 | |
| Chemical compound, drug | D-Glucose | Sigma-Aldrich | G7021 | |
| Chemical compound, drug | Glycerol | Sigma-Aldrich | G5516 | |
| Chemical compound, drug | D-Mannose | Sigma-Aldrich | M2069 | |
| Chemical compound, drug | D-Arabinose | Aladdin | 10323-20-3 | |
| Software, algorithm | MicroCal PEAQ-ITC | Malvern Panalytical |
MicroCal ORIGIN | |
| Software, algorithm | AutoDock Vina | AutoDock Vina | ||
| Software, algorithm | Grahpad prism | Grahpad prism | 8.0.3 | |
| Other | DAPI | Solarbio | C0060 | 1 mg/ml |