Fig. 3.

Linaclotide does not induce swelling of F508del or null intestinal organoids. A: representative images at t = 0 and t = 50 min of intestinal organoids harvested from wild-type (WT), F508del, or null mice following treatment with 10 µM linaclotide. Scale bar = 100 µm. B: total change in area was measured over 50 min and normalized to area at t = 0. Each curve represents the average of at least four wells. C: area under the curve for normalized measurements for each treatment group (baseline set to 100%; *P < 0.01, n ≥ 4 wells).