Fig. 6.

Targeted inhibition of sodium/hydrogen exchanger 3 improves gastrointestinal transit in null mice. A: rates of gastrointestinal transit were measured in adult null mice treated with water, 1 mg/kg tenapanor, 50 µg/kg linaclotide, or both tenapanor and linaclotide. (*P < 0.005, n ≥ 5). B: intestinal organoids were harvested from wild-type (WT) or null mice and treated with either 10 µM linaclotide or 10 µM tenapanor to assess cystic fibrosis transmembrane conductance regulator-dependent swelling. Total change in area was measured over 50 min and normalized to area at t = 0. Each curve represents the average of at least four wells. C: area under the curve for normalized measurements for each treatment group (baseline set to 100%; *P < 0.01, n ≥ 4 wells).