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. 2023 Jan 22;13(3):1059–1075. doi: 10.7150/thno.81067

Figure 7.

Figure 7

Inhibiting increased neuronal autophagy rescues AHN and exerts an antidepressant effect. (A) Representative images of DG in CORT+shScramble and CORT+shAtg5 mice with triple immunostaining of GFAP+ (green), SOX2+ (red), and MCM2+ (gray) cells. (B) Quantification of GFAP+SOX2+ cells. n = 4 mice per group, P = 0.0237. (C) Quantification of GFAP+SOX2+MCM2+ cells. n = 4 mice per group, P = 0.0022. (D) Quantification of GFAP+SOX2+MCM2+/GFAP+SOX2+ cells. n = 4 mice per group, P = 0.0135. (E) Representative images of DG in CORT+shScramble and CORT+shAtg5 mice with double immunostaining of Tbr2+ (red) and MCM2+ (green) cells. (F) Quantification of Tbr2+ cells. n = 3 mice per group, P = 0.4581. (G) Quantification of Tbr2+MCM2+ cells. n = 3 mice per group, P = 0.2891. (H) Quantification of Tbr2+MCM2+/Tbr2+ cells. n = 3 mice per group, P = 0.9484. (I) Representative images of DG in CORT+shScramble and CORT+shAtg5 mice with double immunostaining of DCX+ (red) and MCM2+ (green) cells. (J) Quantification of DCX+ cells. n = 3 mice per group, P = 0.0015. (K) Quantification of DCX+MCM2+ cells. n = 3 mice per group, P = 0.0352. (L) Quantification of DCX+MCM2+/DCX+ cells. n = 3 mice per group, P = 0.3326. (M) Representative images of DG in CORT+shScramble and CORT+shAtg5 mice with triple immunostaining of BrdU+ (green), DCX+ (red), and NeuN+ (gray) cells. (N) Quantification of BrdU+DCX+NeuN+ cells. n = 3 mice per group, P = 0.0050. (O) Quantification of BrdU+DCX-NeuN+ cells. n = 3 mice per group, P < 0.0001. (P) Quantification of BrdU+DCX-NeuN+/BrdU+ cells. n = 3 mice per group, P = 0.0279. Scale bar = 20 μm. (Q) Timeline of the experimental procedure to knock down Atg5 via AAV-mediated shRNA expression. (R) Immobility time in the TST. n = 11 mice per group, P = 0.0414. (S) Immobility time in the FST. n = 11 mice per group, P = 0.0203. (T) Preference index in the exploration stage of the NOR test. CORT+shScramble (n=8 mice per group), CORT+shAtg5 (n=7 mice per group), P < 0.0001. Data are presented as the mean ± SEM. Two-tailed unpaired t-test was used to identify statistically significant differences between datasets (*P < 0.05, **P < 0.01, ***P < 0.001 compared to CORT+shScramble group). n.s., non-significant difference.