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. 2023 Jan 10;13(3):873–895. doi: 10.7150/thno.77444

Figure 2.

Figure 2

NQO1 regulates cell cycle progression at the G2/M phase in cancer cells. (A-B) RKO/pshCont and RKO/pshNQO1 cells (A) and MDA-MB-231/pNQO1 and MDA-MB-231/pCont cells (B) were synchronized at the G1/S boundary via double-thymidine blocking, subsequently released from synchronization, and analyzed for DNA content using flow cytometry. Experiments were conducted in triplicate. Data shown are representative of a typical experiment. AS indicates asynchronization. (C-D) RKO/pshCont and RKO/pshNQO1 cells (C) and MDA-MB-231/pNQO1 and MDA-MB-231/pCont cells (D) were synchronized at the G1/S boundary via double-thymidine blocking and released from synchronization. After incubation for the indicated times, cell lysates were subjected to immunoblot analysis using anti-cyclin B1, anti-NQO1, and anti-β-actin antibodies. AS indicates asynchronization. (E-F) RKO/pshCont and RKO/pshNQO1 cells (E) and MDA-MB-231/pNQO1 and MDA-MB-231/pCont cells (F) were synchronized at the G1/S boundary via double-thymidine blocking, released from synchronization and harvested at the indicated times. CDK1 kinase activities were analyzed using the CDK1 kinase assay kit. All data are presented as mean ± SEM. **** P < 0.0001 with ANOVA. (G-H) RKO/pshCont and RKO/pshNQO1 cells (G) and MDA-MB-231/pNQO1 and MDA-MB-231/pCont cells (H) were synchronized at the G2/M boundary via nocodazole blocking, released from synchronization and analyzed for DNA content using flow cytometry. Experiments were conducted in triplicate. Data are representative of a typical experiment. As indicates asynchronization.