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. 2023 Feb 14;14:820. doi: 10.1038/s41467-023-36445-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Representative immunofluorescence for G3BP1 and eIF2a-pS51 (left) or puromycin (right) in U2OS cells infected with SeV (MOI = 1, 24hpi). SG positive cells are marked with a dotted outline. Scale bar: 5 μm b Quantification of puromycin negative cells (%) in U2OS-A3B-Flag and U2OS-A3B-Flag PKR KO cells infected with SeV (MOI = 1, 24hpi). Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. c Quantification of puromycin negative cells (%) in U2OS-A3B-flag cells ± DOX infected with SeV at 24hpi. Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. d U2OS cells were transfected with siRNA against A3B for 40 h and subsequently infected with SeV at MOI = 1. At 24hpi, the levels of puromycin negative cells (%) were quantified by immunofluorescence. Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. e U2OS-A3B-Flag WT or PKR KO cells ± DOX were infected with SeV at MOI = 1. At 24hpi, the levels of puromycin negative cells (%) were quantified by immunofluorescence. Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. f Quantification of puromycin negative cells (%) in U2OS-A3B-flag cells ± DOX transfected with the indicated siRNA for 40 h and subsequently infected with SeV (MOI = 1, 24hpi). Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. g U2OS-A3B-Flag cells ± DOX were infected with PV at MOI = 1. At 16hpi, the levels of puromycin negative cells (%) were quantified by immunofluorescence. Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. h Quantification of puromycin negative cells (%) in U2OS cells knockdown for A3B and infected with PV (MOI = 1, 16hpi). Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. Source data are provided as a Source Data file.