Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 14;14:820. doi: 10.1038/s41467-023-36445-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, b U2OS cells knocked down for 40 h with the indicated siRNAs were infected with SeV (MOI = 1, 24hpi) and the levels of PKR-pT446 and the indicated proteins were analyzed by western blotting. U2OS cells knocked down with a siRNA control (siCTL) or against PABPC1 for 40 h were infected with PV at MOI = 1 for 20 h (c) or transfected with 200 ng/mL poly(I:C) for 8 h (d). The levels of PKR-pT446, PABPC1, and vinculin were analyzed by western blotting. U2OS cells were transfected with siRNA against PABPC1 for 40 h and subsequently infected with SeV at MOI = 1 (e) or PV at MOI = 1 (f). At 24 hpi (SeV) or 16hpi (PV), the levels of puromycin negative cells (%) were quantified by immunofluorescence. Mean values ± SD (Number of biological replicates, n = 3). P-values were calculated with a two-tailed Welch t-test. g–i. U2OS cells knocked down with a siRNA control (siCTL) or against ADAR1 were infected with SeV at MOI = 1 for 24h. The levels of PKR-pT446 and the indicated proteins were analyzed by western blotting (g), and the number of G3BP1 foci in individual cells. (Red lines indicate the mean (Number of cells, n = 500)), (h) and the puromycin negative cells (Mean values ± SD (Number of biological replicates, n = 3)) (i) were quantified by immunofluorescence. P-values were calculated with a two-tailed Welch t-test. j, k U2OS cells knocked down with the indicated siRNAs were infected with SeV (MOI = 1, 24hpi) and the levels of PKR-pT446 were analyzed by western blotting. l U2OS-A3B-flag cells ± DOX and knocked down with siCTL or siADAR1 for 40 h were infected with SeV (MOI = 1, 24hpi) and the levels of PKR-pT446 and the indicated proteins were analyzed by western blotting. Source data are provided as a Source Data file.