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. 2023 Feb 14;14:820. doi: 10.1038/s41467-023-36445-9

Fig. 8. APOBEC3B prevents the degradation of stress granule-associated RNAs by RNase L.

Fig. 8

a Poly(A), G3BP1, and A3B localization were monitored by sequential IF-FISH (Fluorescence in situ hybridization) in U2OS-A3B-flag cells ± DOX after transfection with poly(I:C) (200 ng/mL, 16 h). Scale bar: 5 μm. b Quantification of poly(A) intensity in G3BP1 foci (arbitrary units) from the experiment described in (a). Red lines indicate the mean (Number of G3BP1 foci, n = 300). P-values were calculated with a two-tailed Welch t-test. c A3B, AHNAK mRNA, G3BP1 localization were monitored by sequential IF-FISH in U2OS-A3B-flag cells ± DOX after transfection with poly(I:C) (200 ng/mL, 16 h). Scale bar: 5 μm. d Quantification of AHNAK mRNA intensity (arbitrary units) in G3BP1 foci after the treatment described in (c). Red lines indicate the mean (Number of G3BP1 foci, n = 300). P-values were calculated with a two-tailed Welch t-test. Poly(A) intensity (arbitrary units) in 200 G3BP1 foci was quantified in U2OS-A3B-flag cells ±DOX subsequent to SeV infection (MOI = 1, 24 hpi) (Number of G3BP1 foci, n = 200) (e) or SINV infection (MOI = 1, 24h) (Number of G3BP1 foci, n = 300) (f). Red lines indicate the mean. P-values were calculated with a two-tailed Welch t-test. g U2OS-A3B-flag cells + DOX were knocked down with indicated siRNAs for 40 h and transfected with poly(I:C) (200 ng/mL, 16 h). The intensity of poly(A) (arbitrary units) in G3BP1 foci was quantified. Red lines indicate the mean (Number of G3BP1 foci, n = 150). P-values were calculated with a two-tailed Welch t-test.