Fig. 3.

ANXA1 (Ac2-26) decreased the levels of apoptosis-related proteins in the kidney tissue of CLP-induced mice. The apoptosis-related protein levels were detected by a western blot analysis. Representative western blots and quantification results of cleaved caspase-3, cleaved caspase-8 and FADD after CLP for 24 h. GAPDH was used as a loading control for the total protein. Note: Ac: ANXA1 (Ac2-26) (1 mg/kg), WRW4 (1.8 mg/kg); Con: control group; CLP: CLP group; CLP + Ac: CLP + Ac2-26 group; CLP + Ac + WRW4: CLP + Ac2-26 + WRW4 group. The data are represented as the mean ± SEM. The data were independently replicated in triplicate. **P < 0.01, ***P < 0.001