Fig. 5.

ANXA1 (Ac2-26) inhibits the production of inflammatory cytokines in LPS-induced HK-2 cells. A The effects of different concentrations of LPS and LPS (10 µg/ml) at different times on the activity of HK-2 cells were detected by CCK-8; B, C effect of Fpr2-siRNA-transfected HK-cells on Fpr2 and the effect of ANXA1 on the expression levels of inflammatory cytokines in HK-2 cells were detected by a western blot analysis; D TNF-α, IL-1β and IL-6 levels in culture supernatants were determined by ELISA after LPS (10 µg/ml) for 24 h. Note: LPS (10 μg/mL), Ac: ANXA1 (Ac2-26) (0.5 μmol/L); Con: control group, LPS: LPS group, LPS + Ac: LPS + Ac2-26 group, LPS + Ac + Fpr2-siRNA: LPS + Ac2-26 + Fpr2-siRNA. The data are represented as the mean ± SEM. The data were independently replicated in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001