Fig. 6.

ANXA1 (Ac2-26) reduces LPS-induced apoptosis-related protein expression in HK-2 cells via the Fpr2 receptor. Apoptosis-related protein levels were detected by a western blot analysis. Representative western blots and quantification results of pro-apoptosis (cleaved caspase-3, cleaved caspase-8 and Bax) and anti-apoptosis (Bcl-2) after LPS (10 µg/ml) for 24 h. GAPDH was used as a loading control for the total protein. Note: LPS (10 μg/mL), Ac: ANXA1 (Ac2-26) (0.5 μmol/L); Con: control group, LPS: LPS group, LPS + Ac: LPS + Ac2-26 group, LPS + Ac + Fpr2-siRNA: LPS + Ac2-26 + Fpr2-siRNA. The data are represented as the mean ± SEM. The data were independently replicated in triplicate. ***P < 0.001