Fig. 8.

Effect of compound IIIc on the production of proinflammatory cytokines by LPS-activated BV-2 cells. Cells were pretreated with 6.25 μM IIIc 1 h prior to incubation of LPS (1 μg/ml) for 24 h. The release of IL-1β (A), IL-6 (B) and TNF-α (C) in the cell culture supernatant were analysed using ELISA as described in Material and Methods. Results are expressed as mean ± SD for each group from three independent experiments. Statistical analysis by one-way ANOVA showed significant differences between the groups (α $=$ 0.05) and was followed by Fisher’s LSD test. Data indicated with ***p $<$ 0.001, (versus vehicle-treated cells) and &&&p $<$ 0.001 (versus LPS-treated cells) reflects statistically significant differences between control and experimental groups