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. 2023 Feb 13;13:2528. doi: 10.1038/s41598-023-28045-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Employing E-Cadherin-mGFP- and mGFP-CD10-based reporters to test specific protein regions. (A) E-Cadherin belongs to the Type-I transmembrane family of proteins. E-Cadherin-mGFP (monomeric GFP) displays strong PM localization in transiently transfected HEK293 cells (white arrows), similar to the AGR system. E-Cadherin-mGFP reporter could be used to re-test intracellular loop domains and termini. (B) CD10 belongs to the family of Type-II transmembrane proteins. mGFP-CD10 shows intense PM localization in HEK293 cells. mGFP-CD10 reporter could be used to test extracellular loop domains and termini. Scale bars: 8 µm.