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. 2023 Feb 13;13:2528. doi: 10.1038/s41598-023-28045-w

Figure 3.

Figure 3

E-Cadherin- and CD10-based reporters confirm intracellular retention quality of the extracellular loop 1, LHFPL547–96. (A) N-terminus of LHFPL5, residues LHFPL51–23, when fused to the C-terminus of E-Cadherin-mGFP does not preclude reporter PM localization, confirming result obtained with AGR. (B) When extracellular loop 1 fragment LHFPL547–96 was fused to C-terminus of mGFP-CD10 reporter, no detectable PM labeling was observed. These results confirm the data acquired using AGR, corroborating the ability of the LHFPL547–96 to halt trafficking to the PM. (C–E) Intracellular loop 1 fragment LHFPL5120–125 (C), extracellular loop 2 fragment LHFPL5149–176 (D), and C-terminus fragment LHFPL5200–219 (E) did not prevent corresponding reporters E-Cadherin-mGFP-LHFPL5120–125 (C), mGFP-CD10-LHFPL5149–176 (D), and E-Cadherin-mGFP-LHFPL520200–219 (E) trafficking to the PM, confirming the result obtained by AGR. Scale bars: 10 µm.