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. 2023 Feb 13;13:2528. doi: 10.1038/s41598-023-28045-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Further scanning ECL1 region LHFPL5^47–96 to identify the exact fragment that prevents trafficking to the PM. (A) ECL1 fragment LHFPL5^47–62 does not prevent reporter trafficking to the PM. When HEK293 cells were transfected with AQP3-tGFP-LHFPL5^47–62, robust PM localization was observed (arrows). (B–D) Likewise, ECL1 fragments LHFPL5^59–74 (B), LHFPL5^71–86 (C), and (D) LHFPL5^83–96 did not prevent PM localization (arrows). Scale bars: 8 µm.