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. 2023 Feb 13;13:2528. doi: 10.1038/s41598-023-28045-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assessing Transmembrane Segments of LHFPL5 for the ability to halt trafficking to the PM using AGR. (A) The first TM segment comprising residues of LHFPL5^16–45 does not prevent reporter trafficking to the PM. PM localization was detected in HEK 293 cells transfected with AQP3-tGFP-LHFPL5^16–45 (arrows). (B–D) Second TM segment amino acid sequence LHFPL5^95–121 (B), third TM sequence LHFPL5^126–154 (C) and fourth TM sequence LHFPL5^180–201 (D) also did not prevent PM localization. Scale bars: 10 µm.