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. 2023 Feb 13;6:170. doi: 10.1038/s42003-023-04536-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — 3D rendering of a Mosaic 4 acquisitions of a ROI in the vicinity of the human primary visual cortex (left top). Orthogonal views of 50 µm MIPs (XY: blue, XZ: green, YZ: red) shows cortical layers independent of the orientation (white arrows). Jagged edges in XZ view result from deskewing when the edge of the acquisition is inside the tissue. Magnified ROIs of the MIP (XY: yellow, XZ: magenta, YZ: cyan) demonstrate qualitatively the isotropically sampled resolution and image and labelling quality deep within the sample. Scale bars: Volume rendering, XY, and YZ plane 3 mm; XZ: 1.5 mm; magnified ROIs 0.5 mm, respectively.