Fig. 6. Two prostate cancer samples cleared and labelled with MASH and imaged with ct-dSPIM Mosaic 16.
Sample are shown at various stages during the processing pipeline on the left side: After deparaffinisation and bleaching (a, e), after staining (b, f), and after RI-matching (i.e., cleared, c, g). On the right side, a MIPs from the Mosaic 16 data set over approx. 50 µm are shown in inverted greyscale (d, h). The red circles indicate the cancerous regions (as identified by a pathologist). Abbreviations: AFS anterior fibromuscular stroma, CZ central zone, U (prostatic) urethra, PZ peripheral zone, TZ transition zone. Scale bars: 3 mm.