Figure 4.

Gapmer ASO 15 improves anxiety-like behavior of hTDP-43 mice in the light and dark transition test

(A–E) WT and hTDP-43 mice were injected with either control ASO C2 or ASO 15 (200 μg) into the cerebral ventricles at 6 weeks of age, and analyzed using the light and dark transition test at 4 and 6 months of age. The representative tracking data of mouse movement at 4 months of age are shown in (A). Time spent in the light compartment (time in lit) (B), distance traveled in the light compartment (distance in lit) (C), number of transitions (D), and latency to the first transition to the light compartment (E) were measured and are shown as bar graphs. Data are presented as the mean ± SEM of 17–22 animals in each group. Statistical analyses were performed to assess differences among groups at each time point by two-way repeated-measures ANOVA followed by the Tukey multiple comparisons test (B–E). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; n.s., not significant.