FIGURE 2.

EV preparations characterisation and quality control. (a) Electron microscopy micrographs of EV samples isolated by HFD. (b) Representative NTA profiles of uEV total RNA. (c) Diameter mean per cell type EV evaluated by NTA. (d) Particle yield per ml of cell free culture media as measured by NTA. (e) Western blotting. Immunodetection of EV markers (TSG101, CD63 and CD81). (f) Representative RNA electropherograms obtained by Agilent RNA 6000 Pico Kit. Black arrows: extracellular vesicles. GEC, glomerular endothelial cells; EV, extracellular vesicles; MC, mesangial cells; NTA, nanoparticle tracking analysis; POD, podocytes; PTC, proximal tubule cells