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Functional studies of proteins encoded by different TCS1 alleles and comparison of phylogenetic trees of N-methyltransferases. A Activity assay results of TCS1 recombinant proteins (n = 3). B Phylogenetic tree of N-methyltransferase coding region sequences related to purine alkaloids in Camellia (red), Coffea (yellow), and Theobroma (blue) is performed by the MEGA Program. Green, blue, and red groups indicate TS, CS, and TcS (theacrine synthase gene) classes.
