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Expression level analysis of TCS1 and TcS in 27 accession tea plants with diverse genetic background. A Purine alkaloid contents in 27 accessions. B–D Determination of TCS1 allele expression levels in 27 accessions of tea plants by qRT-PCR. Primer pairs TCS1-TS, TCS1-CS, and TcS were used to detect gene whose encoded protein had only theobromine synthase (TS), both TS and caffeine synthase (CS), and TcS (theacrine synthase) activities, respectively.
