Figure 4.
Cis-acting elements and promoter activity analysis of different TCS1 alleles. A Distribution of cis-acting elements across the promoter sequences of different TCS1 alleles. Predictive analysis with online software PlantCARE. B GUS histochemical staining of T3 generation Arabidopsis thaliana transduced with different promoters of TCS1 alleles. Red circles indicate important variations in the light-response element. C Determination of the promoter activity of different TCS1 alleles by dual-luciferase assay (n = 6); the difference in promoter activity was determined by detecting the ratio of firefly luciferase and renilla luciferase activities with an empty vector as the control; ***P < 0.001. EV, empty vector.