Figure 5.

Promoter activity analysis of the key regions and cis-elements of truncate promoters of TCS1a. A Distribution of cis-acting elements on the TCS1a promoter sequence with deletions of different lengths. B GUS histochemical staining of T₃ generation of Arabidopsis thaliana transfected with 5′ end fragment deletion of TCS1a promoter. C Determination of the promoter activity of different deleted fragments of the TCS1a promoter (n ≥ 6); ***P < 0.001, **P < 0.01. D Schematic representation of large indel mutations in TCS1e and TCS1f and the absence of a G-Box cis-element. E Comparison of GUS histochemical staining between TaP6 and mutant promoter. TaP6 represents the 331 bp of the TCS1a promoter, TeP6 and TfP6 represent the corresponding promoter sequences of TCS1e and TCS1f, and TaP6m represents a G-Box element near the 3′ end mutated with one base (G-214A). EV indicates empty vector.