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. 2023 Jan 3;120(2):e2211977120. doi: 10.1073/pnas.2211977120

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Copyright © 2023 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 1. — Supernatants from LrS235, but not from LrGusA, block Kv1.3 currents and inhibit the proliferation of human CCR7⁻ T_EM cells. (A) Representativewhole-cell recordings of L929 cells stably expressing mKv1.3 before (control) and after the addition of supernatants diluted 1/10 of LrGusA or LrS235. (B) Percentage of remaining mKv1.3 currents after addition of LrGusA () or LrS235 () supernatants diluted 1/10. Mean ± SEM, each data point represents a different measurement. (C) Representative flow cytometry plots of CellTrace Violet dye dilution and CCR7 expression of CD3⁺ cells from human peripheral blood MNC stimulated for 7 d without any bacterial supernatants (Left) or in the presence of supernatants from LrGusA (Middle) or LrS235 (Right). (D) Percent of divided human CCR7⁻ T_EM and CCR7⁺ naïve/T_CM cells in the absence of stimulation () and after anti-CD3 induced stimulation in the presence of Lr medium (■) or supernatants of LrGusA () or LrS235 () diluted 1/100. Mean ± SEM, N = 4 different buffy coat donors. *P < 0.05, **P < 0.01.

Inline graphic — Supernatants from LrS235, but not from LrGusA, block Kv1.3 currents and inhibit the proliferation of human CCR7⁻ T_EM cells. (A) Representativewhole-cell recordings of L929 cells stably expressing mKv1.3 before (control) and after the addition of supernatants diluted 1/10 of LrGusA or LrS235. (B) Percentage of remaining mKv1.3 currents after addition of LrGusA () or LrS235 () supernatants diluted 1/10. Mean ± SEM, each data point represents a different measurement. (C) Representative flow cytometry plots of CellTrace Violet dye dilution and CCR7 expression of CD3⁺ cells from human peripheral blood MNC stimulated for 7 d without any bacterial supernatants (Left) or in the presence of supernatants from LrGusA (Middle) or LrS235 (Right). (D) Percent of divided human CCR7⁻ T_EM and CCR7⁺ naïve/T_CM cells in the absence of stimulation () and after anti-CD3 induced stimulation in the presence of Lr medium (■) or supernatants of LrGusA () or LrS235 () diluted 1/100. Mean ± SEM, N = 4 different buffy coat donors. *P < 0.05, **P < 0.01.