Fig. 4.

ShK-235, delivered via injection or LrS235, is not immunogenic A. ELISA plates were coated with 10 μg/mL of either porcine collagen II, ShK-235, or HsTX1[R14A]. Sera from rats with CIA treated with vehicle, ShK-235, LrGusA, or LrS235 were tested at tenfold dilution steps starting from a 1:100 dilution. The serum from non-immunized rats was used as a negative control. Each column represents the mean OD of four individual rat sera ± SEM. B. Data from different PBMC donors were normalized to the unstimulated cells with 100% CellTrace Violet. The higher dilution of CellTrace Violet indicates a higher division rate. Data are present as mean ± SEM.