Fig. 3.

CQ blocks the last stage of the CASA pathway and rescues VP40 VLP egress. (A) Schematic diagram of CQ blocking the autophagy flux to rescue VP40 VLP budding. (B–E) eVP40 and mVP40 VLP budding assays and VLP quantification (bar graphs) from HEK293T cells exogenously expressing BAG3 and in the absence or presence of CQ. Statistical significance was analyzed by one-way ANOVA. ns: not significant, *P < 0.05, ***P < 0.001, ****P < 0.0001. (F and G) eVP40 and mVP40 levels were quantified by NIH Image-J from the cytosol or PM fractions of HEK293T cells under the indicated conditions. β-actin and Na/K ATPase served as loading controls for the cytosol and PM fractions, respectively.