Fig. 6.

mTOR inhibitors rapamycin and BEZ-235 restrict egress of infectious EBOV. (A–D) Detection and quantification of phosphorylation levels of mTOR in Huh7 cells incubated with VSV-WT or VSV-eGP (A and B) and eVP40 VLPs or eVP40+eGP VLPs (C and D). Statistical significance was analyzed by one-way ANOVA. ns: not significant, ***P < 0.001. (E) Schematic timeline of EBOV-GFP infection protocol and virus harvest. (F) Confocal images and quantification (bar graph) of EBOV-GFP infected Huh7 cells at 14 h.p.i. in the absence or presence of rapamycin or BEZ-235. (G) Confocal images and quantification (bar graph) of Vero cells infected with EBOV-GFP-containing supernatants that were harvested from Huh7 cells under the indicated conditions (Scale bar, 50 µm.)