Fig. 3.

Survival of the various Cryptococcus neoformans strains and isolates determined by application of heat-ramp cell death stimulus (H–R). The cockatoo passage brain isolates (CPB) recovered from mice infected with the cockatoo strain (CU) were more resistant to cell death when compared with the original cockatoo strain (CU). (A) Survival after a cell death stimulus [10 min linear 30 °C to 56 °C H-R] was determined by CFUs on SAB agar plates incubated for 2 d at 30 °C. (B) Quantification of survival for (A) calculated as log CFU/mL of untreated and heat-ramp–treated strains from four independent experiments. Statistical significance was determined by ordinary one-way ANOVA with Dunnett’s multiple comparisons test, *P = 0.0239. (C) Growth of the same isolates (no heat-ramp) spotted on SAB and incubated for 2 d at 37 °C (C) in two independent experiments; no differences between isolates were detected.