Fig. 4.

Comparison of Cryptococcus neoformans cockatoo, human, and mouse-passaged derivatives' growth at 30 °C and 37 °C. (A) Growth curves of Cryptococcus neoformans strains (CU, PU, CPB1, and CPL1) cultured in Sabouraud dextrose broth at the indicated temperatures plotted as the mean of three biological replicates. (B) Comparison of growth rates at the two temperatures expressed as a ratio of linear phase slopes indicates a significant growth advantage at 37 °C for the mouse-passaged brain isolate compared with the other strains and isolates. (C) Both the patient strain and mouse-derived isolates show a significant decrease in the length of lag time at 37 °C compared with 30 °C. Statistical significance was determined using an ordinary one-way ANOVA (ns = not significant, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001).