Fig. 7.

Interactions with phagocytic cells. (A) Nonlytic escape frequency of each strain during BMDM infection. CPB was the only isolate with a significantly increased frequency of nonlytic escape compared with the original CU strain. *P < 0.05 via a test of equal proportions with Bonferroni correction. Normalized CFU counts of passaged or unpassaged Cryptococcus neoformans isolates or strains following coculture with (B) A. castellanii in A.c. buffer or (C) culture in A.c. buffer alone for 0, 24, and 48 h. Data were normalized to the average CFU count at 0 h for each of the three biological replicates for each strain. Bars represent the mean normalized CFU count with 95% CI. n = 9 for PU and CU experiments and n = 18 for CPB + CPL experiments; mouse-passaged strains (CPB and CPL) were combined to increase statistical power and compare the overall effect of mouse passage with that of PU and CU. *P < 0.05 and **P = 0.0019.