Fig. 1.

Decreased lysoplasmalogens in LXR-stimulated macrophages. A: A schematic overview of the experiment. BMDMs were isolated from wild-type mice (N = 5/group) and stimulated with 1 μM GW3965 (LXR agonist) or vehicle control for 6 h or 16 h for transcriptomic and lipidomics analysis, respectively. Lipid composition was measured using lipidomic profiling and transcriptional profiling was obtained by RNAseq. The total abundance of (B) cholesterol esters (CEs), triglycerides (TGs), and diglycerides (DGs) is shown. C: Lysoplasmalogens are a class of ether lipids uniquely containing an alkenyl bond at the sn-1 position. Lysoplasmalogens are derived from plasmalogens after enzymatic release of the sn-2 conjugated fatty acid. Release of the sn-1 linked fatty aldehyde requires the specific activity of a lysoplasmalogenase. D: Alkenyl PE or PC (PE’ and PC’, respectively) and alkenyl lyso PE or PC (LPE’ and LPC’, respectively) were quantified and total amounts shown. Box plots show the mean ± SEM. ∗ P<0.05, ∗∗ P<0.01.