Fig. 2.

TMEM86a expression is increased by LXR activation and is associated with lipid accumulation in human plaque macrophages. A, B: Volcano plot of differential gene expression from BMDM (N = 4/group) stimulated with 1 μM GW3965 or vehicle for 6 h and corresponding results for Abca1, Idol, Tmem86a, and Tmem86b. C: scRNAseq of carotid plaques from 46 endarterectomy patients was performed, and macrophage clusters were identified. D: TMEM86a expression in individual cells is indicated as Zero: no TMEM86a expression. Low: bottom 25th percentile. Medium: between 25th and 75th percentile. High: Over 75th percentile of TMEM86a expression. E: TMEM86a expression is indicated in each macrophage cluster. ∗∗∗∗ P<0.0001.