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. 2022 Dec 31;64(2):100325. doi: 10.1016/j.jlr.2022.100325

Fig. 3.

Fig. 3

Tmem86a is a transcriptional target of LXR in macrophages and microglia. A: Cells were treated with the LXR agonists 1 μM GW3965 for 6 h or 1 μM T09 for 24 h. B: Cells were cultured for 16 h in the presence (+) or absence (-) of sterols before stimulation with 1 μM GW3965 or 5 μM 22R-hydroxycholesterol. A, B: Expression of Abca1 and Tmem86a was measured by qPCR in (A) BMDM and microglia or in (B) thioglycolate-elicited peritoneal macrophages (PMs). Note: Expression of Tmem86b was undetectable in these experiments. C: Meta-analysis of Tmem86a and Tmem86b expression in LXR wild-type and KO cells in GSE118656. D: ATAC-seq, H3K27Aac ChIP, and LXR ChIP-seq of the Tmem86a locus in (un)treated BMDM. The datasets GSE109965 and GSE79423 were analyzed. E: ChIP-seq (FLAG) was conducted on immortalized LXRα/β(-/-) BMDM macrophages or those stably overexpressing flag-tagged LXRα or LXRβ. The RXRα lane was extracted from the sequence read archive (SRR1514119). Corresponding traces for the Tmem86a locus are shown. Mean ± SEM are depicted. ∗ P<0.05, ∗∗ P<0.01, ∗∗∗ P<0.001, ∗∗∗∗ P< 0.0001.