Fig. 5.

TMEM86a has lysoplasmalogenase activity. A, B: HEK293T cells were transfected with the indicated mouse or human TMEM86a constructs. Crude membrane fractions were isolated, and the rate of LPE’ and LPC’ hydrolysis was determined biochemically in vitro. C: The indicated mutations were introduced in murine TMEM86a and LPE’ and LPC’ hydrolysis determined as above. The mean ± SEM change in absorbance at 340 nM from three independent experiments is plotted. ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.