FIGURE 3.

Assessment of R18 peptide binding to wild‐type and nitrated 14‐3‐3 forms. (a) Crystal structure of engineered R‐18 peptide (pink) bound to 14‐3‐3 (green) (PDB ID: 1A38) showing that this peptide binds the amphipathic groove where phosphorylated peptides and proteins bind. (b) SDS‐PAGE analysis of pull downs in which the indicated biotinylated SUMO‐14‐3‐3 variants were first preincubated with and without sfGFP‐R18 and then immobilized onto streptavidin beads. The beads were washed extensively and then 14‐3‐3 (along with any interacting sfGFP‐R18 protein) was eluted with SUMO protease.