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Analysis of nitrated 14‐3‐3 interactions with proteins from HEK293T cell lysate. Sepharose‐conjugated 14‐3‐3 variants (or Sepharose conjugated to Tris; “No bait”) were incubated with HEK293T lysates, after which resins were collected, washed and then the interacting proteins were eluted with denaturant. Gel was stained with Coomassie Blue. “Input” lane is the HEK293T lysate prior to incubation with 14‐3‐3 variants.
