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. 2023 Feb 13;13:29. doi: 10.1186/s13578-023-00982-z

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Histology analysis confirms the accuracy of echocardiography assessment for nonperfect regeneration with the adhesion of injured X. tropicalis hearts. A1: Representative H&E staining of regenerated hearts at 45 daar; echocardiography assessment identified regeneration in a nonperfect manner with adhesion. A2: 5 × of dotted rectangle area of A1. A3: 2.4 × of dotted rectangle area of A2.. B1: Representative Masson’s trichrome staining of a regenerated heart at 45 daar; echocardiography assessment identified regeneration in a nonperfect manner with adhesion. B2: 5 × of dotted rectangle area of B1. B3: 2.4 × of dotted rectangle area of B2. H&E staining showed that the amputated heart was regenerated with a nearly heart-shaped morphology containing a defect after the adhesion was cleaned. In addition, Masson’s trichrome staining revealed fibrotic structures in the regenerated myocardium. As the adhesion between the regenerated area and peripheral tissue was cleaned when the regenerated hearts were isolated, the fibrotic tissue of the adhesion could not be stained