Table 1.

Primers Used for Real-Time PCR

Gene Name	Primer Sequence
HMGB1	F: 5’-ATGCTTCAGTCAACTTCTCAGA-3’; R: 5’-CATTTCTCTTTCATAACGGGCC-3’
TNF-α	F: 5’-GAGGCCAAGCCCTGGTATG-3’; R: 5’-CGGGCCGATTGATCTCAGC-3’
IL-6	F: 5’-ACTCACCTCTTCAGAACGAATTG-3’; R: 5’-CCATCTTTGGAAGGTTCAGGTTG-3’
Twist	F: 5’-GTCCGCAGTCTTACGAGGAG-3’; R: 5’-GCTTGAGGGTCTGAATCTTGCT-3’
Slug	F: 5’-TGTGACAAGGAATATGTGAGCC-3’; R: 5’-TGAGCCCTCAGATTTGACCTG-3’
GAPDH	F: 5’-ACAACTTTGGTATCGTGGAAGG-3’; R: 5’-GCCATCACGCCACAGTTTC-3’