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. 2023 Feb 14;8:18. doi: 10.1038/s41541-023-00610-4

Fig. 1. A lumazine synthase nanoparticle scaffold enables efficient RBD display.

Fig. 1

a SDS-PAGE analysis under reducing conditions of RBD expressing SpyCatcher (RBD-Catch), lumazine synthase expressing SpyTag (LuS-Tag), RBD nanoparticle (RBD-NP), as well as native RBD and Spike proteins. b, c Transmission electron microscopy (b) and dynamic light scattering (c) analyses of RBD-NP. Scale bar, 50 nm. d–f ELISA plates were coated with RBD, Spike, RBD-NP and LuS-Tag at 1 μg/ml (d), 5 μg/ml (e) or 0.5 μg/ml (f). Binding of recombinant human ACE2 (hACE2) or anti-RBD H4 and CR3022 Ab clones tested at multiple concentrations was expressed as optical density (OD) at 450 nm or area under the curve (AUC). Data are presented as mean and SD. N = 3–6 experiments. *, **, ***, and **** respectively indicate p < 0.05, 0.01, 0.001, and 0.0001. Statistical significance was determined by one-way ANOVA corrected for multiple comparisons. The color code indicates comparisons among experimental groups. PDI: polydispersity index. c–e Source data are provided as Supplementary Data.