FIG 3.
Histopathological analysis of brain samples following H5N8 HPAIV infection. (A) Immunohistochemical anti-NP staining of hematoxylin-counterstained chicken and duck brain sections from moribund animals. Scale bars, 100 μm. (B) Hematoxylin and eosin staining on brain sections from moribund animals. Lymphocytic meningoencephalitis is observed only in the brains of H5N8 HPAIV-infected ducks. The star symbol indicates sites of lymphocytic infiltration of perivascular meningeal spaces, and the triangle indicates neuronal necrosis and the losange focal gliosis. Scale bars, 50 μm.