FIG 1.

Antibiotic susceptibility patterns of S. aureus strains. (A to C) Strains JE2, 117, and 158 were exposed to increasing concentrations of gentamicin (A), cefazolin (B), or bacitracin (C). (A) JE2 displays resistance to gentamicin, with a minimum inhibitory concentration (MIC) of 1.0 μg/mL, while 117 and 158 are susceptible, as they exhibit MICs of 0.5 and 0.25 μg/mL, respectively. (B) JE2, 117, and 158 are all susceptible to cefazolin, with MICs of 1.0, 0.5, and 0.5 μg/mL, respectively. (C) JE2, 117, and 158 are all resistant to bacitracin, as they exhibit MICs of >32, 24, and >32 μg/mL, respectively. The red line represents the MIC breakpoint for each antibiotic for S. aureus. An MIC value at or above the MIC breakpoint classifies the pathogen as resistant to the antibiotic. (D) Susceptibility of S. aureus strains to triple antibiotic pocket irrigant (TAPI) was determined based on an increase in optical density, indicating growth of strains, when exposed to TAPI compared to those not exposed to TAPI. JE2, 117, and 158 display susceptibility to TAPI. MICs were determined using an optical density at 600 nm (OD₆₀₀) threshold lower than 0.1, and heat maps display a representative of one of the three replicates. MHB, Mueller-Hinton broth.